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dc.creatorPérez Martínez, Isabeles_ES
dc.creatorZhao, Youfues_ES
dc.creatorMurillo Martínez, Jesúses_ES
dc.creatorSundin, George W.es_ES
dc.creatorRamos, Cayoes_ES
dc.date.accessioned2019-01-23T11:16:21Z
dc.date.available2019-01-23T11:16:21Z
dc.date.issued2007
dc.identifier.issn0021-9193(Print)
dc.identifier.issn1098-5530 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/32085
dc.description.abstractPseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of the related species Pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. However, there is a general lack of information about the gene content of P. savastanoi pv. savastanoi plasmids and their role in the interaction of this pathogen with olive plants. We designed a DNA macroarray containing 135 plasmid-borne P. syringae genes to conduct a global genetic analysis of 32 plasmids obtained from 10 P. savastanoi pv. savastanoi strains. Hybridization results revealed that the number of PFPs per strain varied from one to four. Additionally, most strains contained at least one plasmid (designated non-PFP) that did not hybridize to the repA gene of pPT23A. Only three PFPs contained genes involved in the biosynthesis of the virulence factor indole-3-acetic acid (iaaM, iaaH, and iaaL). In contrast, ptz, a gene involved in the biosynthesis of cytokinins, was found in five PFPs and one non-PFP. Genes encoding a type IV secretion system (T4SS), type IVA, were found in both PFPs and non-PFPs; however, type IVB genes were found only on PFPs. Nine plasmids encoded both T4SSs, whereas seven other plasmids carried none of these genes. Most PFPs and non-PFPs hybridized to at least one putative type III secretion system effector gene and to a variety of additional genes encoding known P. syringae virulence factors and one or more insertion sequence transposase genes. These results indicate that non-PFPs may contribute to the virulence and fitness of the P. savastanoi pv. savastanoi host. The overall gene content of P. savastanoi pv. savastanoi plasmids, with their repeated information, mosaic arrangement, and insertion sequences, suggests a possible role in adaptation to a changing environment.en
dc.description.sponsorshipThis project was supported by Spanish MCYT grants AGL2002-02214 and AGL-2005-02090 and Junta de Andalucia grant CVI-264 to C.R., Spanish MCYT grant AGL2004-03143 to J.M., U.S. Department of Agriculture grant USDA-NRI 2003-01531 to G.W.S., and the Agriculture Experimental Stations of Illinois and Michigan. I.P.-M. was awarded a Spanish MEC fellowship AP2002-3800 and an EMBO short-term fellowship (ASTF 225.00-05).en
dc.format.extent11 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofJournal of Bacteriology, vol. 190, nº 2, jan. 2008, p. 625–635en
dc.rights© 2008, American Society for Microbiology. All Rights Reserved.en
dc.subjectPseudomonas savastanoien
dc.subjectSavastanoi Plasmidsen
dc.subjectGenomicen
dc.titleGlobal genomic analysis of Pseudomonas savastanoi pv. savastanoi plasmidsen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/jb.01067-07
dc.relation.publisherversionhttps://doi.org/10.1128/jb.01067-07
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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