The mbo operon is specific and essential for biosynthesis of mangotoxin in Pseudomonas syringae
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Fecha
2012Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.1371/journal.pone.0036709
Resumen
Mangotoxin is an antimetabolite toxin produced by certain Pseudomonas syringae pv. syringae strains. This toxin is an oligopeptide that inhibits ornithine N-acetyl transferase, a key enzyme in the biosynthesis of ornithine and arginine. Previous studies have reported the involvement of the putative nonribosomal peptide synthetase MgoA in virulence and mangotoxin production. In this study, we an ...
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Mangotoxin is an antimetabolite toxin produced by certain Pseudomonas syringae pv. syringae strains. This toxin is an oligopeptide that inhibits ornithine N-acetyl transferase, a key enzyme in the biosynthesis of ornithine and arginine. Previous studies have reported the involvement of the putative nonribosomal peptide synthetase MgoA in virulence and mangotoxin production. In this study, we analyse a new chromosomal region of P. syringae pv. syringae UMAF0158, which contains six coding sequences arranged as an operon (mbo operon). The mbo operon was detected in only mangotoxin-producing strains, and it was shown to be essential for the biosynthesis of this toxin. Mutants in each of the six ORFs of the mbo operon were partially or completely impaired in the production of the toxin. In addition, Pseudomonas spp. mangotoxin non-producer strains transformed with the mbo operon gained the ability to produce mangotoxin, indicating that this operon contains all the genetic information necessary for mangotoxin biosynthesis. The generation of a single transcript for the mbo operon was confirmed and supported by the allocation of a unique promoter and Rho-independent terminator. The phylogenetic analysis of the P. syringae strains harbouring the mbo operon revealed that these strains clustered together. [--]
Materias
Pv. Tomato DC3000,
Artemis comparison tool,
Escherichia coli,
Gene cluster,
Phytotoxin coronatine,
Peptide synthetases,
Genome,
Sequence,
Beta lactam,
Functional characterization,
Phaseolotoxin,
Production
Editor
Public Library of Science
Publicado en
Plos One, 2012, 7 (5): e36709
Notas
UPNa. Departamento de Producción Agraria. Laboratorio de Patología Vegetal
Versión del editor
Entidades Financiadoras
This work has been supported through grants from the Regional Government of Andalucía (Spain), grants from CICE - Junta de Andalucía, Ayudas
Grupo PAIDI AGR-169 and Proyecto de Excelencia (P07-AGR-23 02471), and co-financed by FEDER (EU). Plan Propio of the University of Málaga funded visitation of
VJC to the Public University of Navarra, Spain.