High irradiance increases NH4+ tolerance in Pisum sativum: higher carbon and energy availability improve ion balance but not N assimilation

Ariz Arnedo, Idoia; Artola Rezola, Ekhiñe; Asensio, Aarón C.; Cruchaga Moso, Saioa; Aparicio Tejo, Pedro María; Morán Juez, José Fernando

High irradiance increases NH4+ tolerance in Pisum sativum: higher carbon and energy availability improve ion balance but not N assimilation

dc.contributor.author	Ariz Arnedo, Idoia
dc.contributor.author	Artola Rezola, Ekhiñe
dc.contributor.author	Asensio, Aarón C.
dc.contributor.author	Cruchaga Moso, Saioa
dc.contributor.author	Aparicio Tejo, Pedro María
dc.contributor.author	Morán Juez, José Fernando
dc.contributor.department	Ciencias del Medio Natural	es_ES
dc.contributor.department	Natura Ingurunearen Zientziak	eu
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	en
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.date.accessioned	2025-01-29T19:06:28Z
dc.date.available	2025-01-29T19:06:28Z
dc.date.issued	2011-03-02
dc.date.updated	2025-01-29T18:46:40Z
dc.description.abstract	The widespread use of NO3− fertilization has had a major ecological impact. NH4+ nutrition may help to reduce this impact, although high NH4+ concentrations are toxic for most plants. The underlying tolerance mechanisms are not yet fully understood, although they are thought to include the limitation of C, the disruption of ion homeostasis, and a wasteful NH4+ influx/efflux cycle that carries an extra energetic cost for root cells. In this study, high irradiance (HI) was found to induce a notable tolerance to NH4+ in the range 2.5–10 mM in pea plants by inducing higher C availability, as shown by carbohydrate content. This capacity was accompanied by a general lower relative N content, indicating that tolerance is not achieved through higher net N assimilation on C-skeletons, and it was also not attributable to increased GS content or activity in roots or leaves. Moreover, HI plants showed higher ATP content and respiration rates. This extra energy availability is related to the internal NH4+ content regulation (probably NH4+ influx/efflux) and to an improvement of the cell ionic balance. The limited C availability at lower irradiance (LI) and high NH4+ resulted in a series of metabolic imbalances, as reflected in a much higher organic acid content, thereby suggesting that the origin of the toxicity in plants cultured at high NH4+ and LI is related to their inability to avoid large-scale accumulation of the NH4+ ion.	en
dc.description.sponsorship	This work was supported by the Spanish MICIIN (grant nos. AGL2006-12792-CO2-01 and AGL2009-13339-CO2-02 [to P.A.-T.] and AGL2007-64432/AGR [to J.F.M.]). IA was supported by a doctoral Fellowship from the Public University of Navarre.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/msword	en
dc.identifier.citation	Ariz, I., Artola, E., Cabrera-Asensio, A., Cruchaga, S., Aparicio-Tejo, P.M., Moran, J.F. (2011) High irradiance increases NH4+ tolerance in Pisum sativum: higher carbon and energy availability improve ion balance but not N assimilation. Journal of Plant Physiology, 168(10), 1009-1015. https://doi.org/10.1016/j.jplph.2010.11.022.
dc.identifier.doi	10.1016/j.jplph.2010.11.022
dc.identifier.issn	0176-1617
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/53163
dc.language.iso	eng
dc.publisher	Elsevier
dc.relation.ispartof	Journal of Plant Physiology 168(10), 2011, 1009-1015
dc.relation.projectID	info:eu-repo/grantAgreement/MEC//AGL2006-12792-C02-01/ES/
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//AGL2009-13339-C02-02/ES /
dc.relation.projectID	info:eu-repo/grantAgreement/MEC//AGL2007-64432/ES/
dc.relation.publisherversion	https://doi.org/10.1016/j.jplph.2010.11.022
dc.rights	© 2011 Elsevier Gmbh. This manuscript version is made available under the CC-BY-NC-ND 4.0
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Ammonium nutrition	en
dc.subject	Ammonium tolerance	en
dc.subject	Ammonium toxicity	en
dc.subject	C-N metabolism	en
dc.subject	High irradiance	en
dc.subject	Ion balance	en
dc.subject	Pisum sativum L.	en
dc.title	High irradiance increases NH4+ tolerance in Pisum sativum: higher carbon and energy availability improve ion balance but not N assimilation	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/acceptedVersion
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