Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335

Añorga García, Maite; Urriza Leoz, Miriam; Ramos, Cayo; Murillo Martínez, Jesús

Publication:
Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335

dc.contributor.author	Añorga García, Maite
dc.contributor.author	Urriza Leoz, Miriam
dc.contributor.author	Ramos, Cayo
dc.contributor.author	Murillo Martínez, Jesús
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.date.accessioned	2022-12-22T11:53:36Z
dc.date.available	2022-12-22T11:53:36Z
dc.date.issued	2022
dc.date.updated	2022-12-22T11:40:48Z
dc.description.abstract	Pseudomonas syringae pv. savastanoi NCPPB 3335 is the causal agent of olive knot disease and contains three virulence plasmids: pPsv48A (pA), 80 kb; pPsv48B (pB), 45 kb, and pPsv48C (pC), 42 kb. Here we show that pB contains a complete MPFT (previously type IVA secretion system) and a functional origin of conjugational transfer adjacent to a relaxase of the MOBP family; pC also contains a functional oriT-MOBP array, whereas pA contains an incomplete MPFI (previously type IVB secretion system), but not a recognizable oriT. Plasmid transfer occurred on solid and in liquid media, and on leaf surfaces of a non-host plant (Phaseolus vulgaris) with high (pB) or moderate frequency (pC); pA was transferred only occasionally after cointegration with pB. We found three plasmid-borne and three chromosomal relaxase genes, although the chromosomal relaxases did not contribute to plasmid dissemination. The MOBP relaxase genes of pB and pC were functionally interchangeable, although with di ering eciencies. We also identified a functional MOBQ mobilization region in pC, which could only mobilize this plasmid. Plasmid pB could be eciently transferred to strains of six phylogroups of P. syringae sensu lato, whereas pC could only be mobilized to two strains of phylogroup 3 (genomospecies 2). In two of the recipient strains, pB was stably maintained after 21 subcultures in liquid medium. The carriage of several relaxases by the native plasmids of P. syringae impacts their transfer frequency and, by providing functional diversity and redundancy, adds robustness to the conjugation system.	en
dc.description.sponsorship	This work was funded by the Spanish Plan Nacional I+D+i grants PID2020-115177RB-C21 and PID2020- 115177RB-C22 financed by the Ministerio de Ciencia e Innovación and the Agencia Estatal de Investigación, MCIN/AEI/10.13039/501100011033/, Spain.	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.citation	Añorga M, Urriza M, Ramos C and Murillo J (2022) Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335. Front. Microbiol. 13:1076710. doi: 10.3389/fmicb.2022.1076710	en
dc.identifier.doi	10.3389/fmicb.2022.1076710
dc.identifier.issn	1664-302X
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/44491
dc.language.iso	eng	en
dc.publisher	Frontiers Media	en
dc.relation.ispartof	Frontiers in Microbiology 13:1076710	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115177RB-C21/ES/	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115177RB-C22/ES/	en
dc.relation.publisherversion	https://doi.org/10.3389/fmicb.2022.1076710
dc.rights	© 2022 Añorga, Urriza, Ramos and Murillo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	Cytokinin biosynthesis	en
dc.subject	Type III effectors	en
dc.subject	Olive knot disease	en
dc.subject	Type IV secretion system	en
dc.subject	Mating pair formation	en
dc.subject	Pseudomonas amygdali	en
dc.subject	Pseudomonas savastanoi	en
dc.subject	Conjugation (mating)	en
dc.title	Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335	en
dc.type	Artículo / Artikulua	es
dc.type	info:eu-repo/semantics/article	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dspace.entity.type	Publication
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