Biofilm morphology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) on poly-D,L-lactide-co-poly(ethylene glycol) (PDLLA-PEG) coated titanium

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dc.contributor.authorTurner, Adam Benedict
dc.contributor.authorZermeño-Pérez, David
dc.contributor.authorMysior, Margaritha M.
dc.contributor.authorGiraldo-Osorno, Paula Milena
dc.contributor.authorGarcía Martínez, Begoña
dc.contributor.authorO'Gorman, Elizabeth
dc.contributor.authorOubihi, Shafik
dc.contributor.authorSimpson, Jeremy C.
dc.contributor.authorLasa Uzcudun, Íñigo
dc.contributor.authorÓ'Cróinín, Tadhg
dc.contributor.authorTrobos, Margarita
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.date.accessioned2025-02-19T18:29:02Z
dc.date.available2025-02-19T18:29:02Z
dc.date.issued2024-10-05
dc.date.updated2025-02-19T18:17:06Z
dc.description.abstractBiodegradable polymeric coatings are being explored as a preventive strategy for orthopaedic device-related infection. In this study, titanium surfaces (Ti) were coated with poly-D,L-lactide (PDLLA, (P)), polyethylene-glycol poly-D,L-lactide (PEGylated-PDLLA, (PP20)), or multi-layered PEGylated-PDLLA (M), with or without 1 % silver sulfadiazine. The aim was to evaluate their cytocompatibility, resistance to Staphylococcus aureus biofilm formation, and their potential to enhance the susceptibility of any biofilm formed to antibiotics. Using automated high-content screening confocal microscopy, biofilm formation of a clinical methicillin-resistant Staphylococcus aureus (MRSA) isolate expressing GFP was quantified, along with isogenic mutants that were unable to form polysaccharidic or proteinaceous biofilm matrices. The results showed that PEGylated-PDLLA coatings exhibited significant antibiofilm properties, with M showing the highest effect. This inhibitory effect was stronger in S. aureus biofilms with a matrix composed of proteins compared to those with an exopolysaccharide (PIA) biofilm matrix. Our data suggest that the antibiofilm effect may have been due to (i) inhibition of the initial attachment through microbial surface components recognising adhesive matrix molecules (MSCRAMMs), since PEG reduces protein surface adsorption via surface hydration layer and steric repulsion; and (ii) mechanical disaggregation and dispersal of microcolonies due to the bioresorbable/degradable nature of the polymers, which undergo hydration and hydrolysis over time. The disruption of biofilm morphology by the PDLLA-PEG co-polymers increased S. aureus susceptibility to antibiotics like rifampicin and fusidic acid. Adding 1 % AgSD provided additional early bactericidal effects on both biofilm and planktonic S. aureus. Additionally, the coatings were cytocompatible with immune cells, indicating their potential to enhance bacterial clearance and reduce bacterial colonisation of titanium-based orthopaedic biomaterials.en
dc.description.sponsorshipThis research was funded by the European Commission within the H2020- MSCA grant agreement No. 861046 (BIOREMIA-ETN); Swedish Research Council (2022-00853); the Swedish state under the agreement between the Swedish government and the county councils; the ALF agreement (ALFGBG-978896); the IngaBritt and Arne Lundberg Foundation (LU2021-0048); the Hjalmar Svensson Foundation; the Doctor Felix Neuberghs Foundation; the Adlerbertska Foundation; the Area of Advance Materials of Chalmers/ GU Biomaterials within the Strategic Research Area initiative launched by the Swedish government; the Spanish Ministry of Science, Innovation and Universities grant PID2020-113494RB-I00 (Agencia Española de Investigación / Fondo Europeo de Desarrollo Regional , European Union) to I.L.; and S.O. was funded through the Government of Ireland Postgraduate Scholarship Programme by the Irish Research Council (GOIPG/2023/3290).
dc.format.mimetypeapplication/pdfen
dc.identifier.citationTurner, A. B., Zermeño-Pérez, D., Mysior, M. M., Giraldo-Osorno, P. M., García, B., O'Gorman, E., Oubihi, S., Simpson, J. C., Lasa, I., Ó Cróinín, T., Trobos, M. (2024) Biofilm morphology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) on poly-D,L-lactide-co-poly(ethylene glycol) (PDLLA-PEG) coated titanium. Biofilm, 8, 1-19. https://doi.org/10.1016/j.bioflm.2024.100228.
dc.identifier.doi10.1016/j.bioflm.2024.100228
dc.identifier.issn2590-2075
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/53505
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofBiofilm, 8, 2024, 100228
dc.relation.projectIDinfo:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/861046/
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113494RB-I00/ES/
dc.relation.publisherversionhttps://doi.org/10.1016/j.bioflm.2024.100228
dc.rights© 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license.
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPoly-D,L-lactide (PDLLA)en
dc.subjectPolyethylene-glycol (PEG)en
dc.subjectBiofilmen
dc.subjectMethicillin-resistant Staphylococcus aureus (MRSA)en
dc.subjectAntibioticsen
dc.titleBiofilm morphology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) on poly-D,L-lactide-co-poly(ethylene glycol) (PDLLA-PEG) coated titaniumen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication7f89244d-67ab-4c4d-b868-ad7ae9112055
relation.isAuthorOfPublicationc654d104-1ae2-41cf-9215-4b4bed3e5ea6
relation.isAuthorOfPublication.latestForDiscovery7f89244d-67ab-4c4d-b868-ad7ae9112055

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