The transcriptional landscape of plant infection by the rice blast fungus Magnaporthe oryzae reveals distinct families of temporally co-regulated and structurally conserved effectors

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dc.contributor.authorYan, Xia
dc.contributor.authorTang, Bozeng
dc.contributor.authorRyder, Lauren S.
dc.contributor.authorMacLean, Dan
dc.contributor.authorWere, Vincent M.
dc.contributor.authorEseola, Alice Bisola
dc.contributor.authorCruz-Mireles, Neftaly
dc.contributor.authorMa, Weibin
dc.contributor.authorFoster, Andrew J.
dc.contributor.authorOsés Ruiz, Miriam
dc.contributor.authorTalbot, Nicholas J.
dc.contributor.departmentAgronomía, Biotecnología y Alimentaciónes_ES
dc.contributor.departmentAgronomia, Bioteknologia eta Elikaduraeu
dc.date.accessioned2024-05-22T15:42:37Z
dc.date.available2024-05-22T15:42:37Z
dc.date.issued2023
dc.date.updated2024-05-22T14:51:31Z
dc.description.abstractThe rice blast fungus Magnaporthe oryzae causes a devastating disease that threatens global rice (Oryza sativa) production. Despite intense study, the biology of plant tissue invasion during blast disease remains poorly understood. Here we report a high-resolution transcriptional profiling study of the entire plant-associated development of the blast fungus. Our analysis revealed major temporal changes in fungal gene expression during plant infection. Pathogen gene expression could be classified into 10 modules of temporally co-expressed genes, providing evidence for the induction of pronounced shifts in primary and secondary metabolism, cell signaling, and transcriptional regulation. A set of 863 genes encoding secreted proteins are differentially expressed at specific stages of infection, and 546 genes named MEP (Magnaporthe effector protein) genes were predicted to encode effectors. Computational prediction of structurally related MEPs, including the MAX effector family, revealed their temporal co-regulation in the same co-expression modules. We characterized 32 MEP genes and demonstrate that Mep effectors are predominantly targeted to the cytoplasm of rice cells via the biotrophic interfacial complex and use a common unconventional secretory pathway. Taken together, our study reveals major changes in gene expression associated with blast disease and identifies a diverse repertoire of effectors critical for successful infection.en
dc.description.sponsorshipThis work was supported by the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no. 294702 GENBLAST, the Biotechnology and Biological Sciences Research Council Institute Strategic Programme Grant in Plant Health BBS/E/J/000PR9797 and grant BB/V016342/1, and by an award from The Gatsby Charitable Foundation to N.J.T.en
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dc.format.mimetypeapplication/zipen
dc.identifier.citationYan, X., Tang, B., Ryder, L. S., MacLean, D., Were, V. M., Eseola, A. B., Cruz-Mireles, N., Ma, W., Foster, A. J., Osés-Ruiz, M., Talbot, N. J. (2023) The transcriptional landscape of plant infection by the rice blast fungus Magnaporthe oryzae reveals distinct families of temporally co-regulated and structurally conserved effectors. The Plant Cell, 35(5), 1360-1385. https://doi.org/10.1093/plcell/koad036.en
dc.identifier.doi10.1093/plcell/koad036
dc.identifier.issn1040-4651
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/48145
dc.language.isoengen
dc.publisherOxford University Pressen
dc.relation.ispartofThe Plant Cell 2023, 35(5), 1360-1385en
dc.relation.projectIDinfo:eu-repo/grantAgreement/European Commission/FP7/294702/
dc.relation.publisherversionhttps://doi.org/10.1093/plcell/koad036
dc.rights© The Author(s) 2023. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectMagnaporthe oryzaeen
dc.subjectBlast diseaseen
dc.titleThe transcriptional landscape of plant infection by the rice blast fungus Magnaporthe oryzae reveals distinct families of temporally co-regulated and structurally conserved effectorsen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublicatione3c7965f-41da-43eb-83ef-097f6b78ef83
relation.isAuthorOfPublication.latestForDiscoverye3c7965f-41da-43eb-83ef-097f6b78ef83

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