Iflavirus covert infection increases susceptibility to nucleopolyhedrovirus disease in Spodoptera exigua

Carballo Palos, Arkaitz; Williams, Trevor; Murillo Pérez, Rosa; Caballero Murillo, Primitivo

Iflavirus covert infection increases susceptibility to nucleopolyhedrovirus disease in Spodoptera exigua

dc.contributor.author	Carballo Palos, Arkaitz
dc.contributor.author	Williams, Trevor
dc.contributor.author	Murillo Pérez, Rosa
dc.contributor.author	Caballero Murillo, Primitivo
dc.contributor.department	Agronomia, Bioteknologia eta Elikadura	eu
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.contributor.department	Agronomía, Biotecnología y Alimentación	es_ES
dc.date.accessioned	2020-07-06T08:41:24Z
dc.date.available	2020-07-06T08:41:24Z
dc.date.issued	2020
dc.description.abstract	Naturally occurring covert infections in lepidopteran populations can involve multiple viruses with potentially different transmission strategies. In this study, we characterized covert infection by two RNA viruses, Spodoptera exigua iflavirus 1 (SeIV-1) and Spodoptera exigua iflavirus 2 (SeIV-2) (family Iflaviridae) that naturally infect populations of Spodoptera exigua, and examined their influence on susceptibility to patent disease by the nucleopolyhedrovirus Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) (family Baculoviridae). The abundance of SeIV-1 genomes increased up to ten-thousand-fold across insect developmental stages after surface contamination of host eggs with a mixture of SeIV-1 and SeIV-2 particles, whereas the abundance of SeIV-2 remained constant across all developmental stages. Low levels of SeIV-2 infection were detected in all groups of insects, including those that hatched from surface-decontaminated egg masses. SeIV-1 infection resulted in reduced larval weight gain, and an unbalanced sex ratio, whereas larval developmental time, pupal weight, and adult emergence and fecundity were not significantly affected in infected adults. The inoculation of S. exigua egg masses with iflavirus, followed by a subsequent infection with SeMNPV, resulted in an additive effect on larval mortality. The 50% lethal concentration (LC50) of SeMNPV was reduced nearly 4-fold and the mean time to death was faster by 12 h in iflavirus-treated insects. These results suggest that inapparent iflavirus infections may be able to modulate the host response to a new pathogen, a finding that has particular relevance to the use of SeMNPV as the basis for biological pest control products.	en
dc.description.sponsorship	This research was funded by Spanish Ministry of Science and Innovation (AGL2011-30352-C02-01). AC received a doctoral grant from AGL2011-30352-C02-01.	en
dc.format.extent	12 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.3390/v12050509
dc.identifier.issn	1999-4915
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/37341
dc.language.iso	eng	en
dc.publisher	MDPI	en
dc.relation.ispartof	Viruses, 2020, 12(5), 509	en
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//AGL2011-30352-C02-01/ES/
dc.relation.publisherversion	https://doi.org/10.3390/v12050509
dc.rights	© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Iflavirus	en
dc.subject	SeMNPV	en
dc.subject	Covert infection	en
dc.subject	Spodoptera exigua	en
dc.title	Iflavirus covert infection increases susceptibility to nucleopolyhedrovirus disease in Spodoptera exigua	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
relation.isAuthorOfPublication	e283ed01-c91a-4878-b413-a4251e792835
relation.isAuthorOfPublication	0b5a3470-9d73-4ac7-a923-c4f6079df358
relation.isAuthorOfPublication	46f59760-02e7-4f4d-bfb2-e505b2875f48
relation.isAuthorOfPublication	ecde8e03-14c2-46a3-9e89-ae3b2c668297
relation.isAuthorOfPublication.latestForDiscovery	e283ed01-c91a-4878-b413-a4251e792835