Transcriptome metabolic characterization of tuber borchii SP1-A new spanish strain for in vitro studies of the bianchetto truffle

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dc.contributor.authorChuina Tomazeli, Emilia
dc.contributor.authorAlfaro Sánchez, Manuel
dc.contributor.authorZambonelli, Alessandra
dc.contributor.authorGarde Sagardoy, Edurne
dc.contributor.authorPérez Garrido, María Gumersinda
dc.contributor.authorJiménez Miguel, Idoia
dc.contributor.authorRamírez Nasto, Lucía
dc.contributor.authorSalman, Hesham
dc.contributor.authorPisabarro de Lucas, Gerardo
dc.contributor.departmentInstitute for Multidisciplinary Research in Applied Biology - IMABen
dc.date.accessioned2023-09-22T16:23:53Z
dc.date.available2023-09-22T16:23:53Z
dc.date.issued2023
dc.date.updated2023-09-22T15:52:16Z
dc.description.abstractTruffles are ascomycete hypogeous fungi belonging to the Tuberaceae family of the Pezizales order that grow in ectomycorrhizal symbiosis with tree roots, and they are known for their peculiar aromas and flavors. The axenic culture of truffle mycelium is problematic because it is not possible in many cases, and the growth rate is meager when it is possible. This limitation has prompted searching and characterizing new strains that can be handled in laboratory conditions for basic and applied studies. In this work, a new strain of Tuber borchii (strain SP1) was isolated and cultured, and its transcriptome was analyzed under different in vitro culture conditions. The results showed that the highest growth of T. borchii SP1 was obtained using maltose-enriched cultures made with soft-agar and in static submerged cultures made at 22 °C. We analyzed the transcriptome of this strain cultured in different media to establish a framework for future comparative studies, paying particular attention to the central metabolic pathways, principal secondary metabolite gene clusters, and the genes involved in producing volatile aromatic compounds (VOCs). The results showed a transcription signal for around 80% of the annotated genes. In contrast, most of the transcription effort was concentrated on a limited number of genes (20% of genes account for 80% of the transcription), and the transcription profile of the central metabolism genes was similar in the different conditions analyzed. The gene expression profile suggests that T. borchii uses fermentative rather than respiratory metabolism in these cultures, even in aerobic conditions. Finally, there was a reduced expression of genes belonging to secondary metabolite clusters, whereas there was a significative transcription of those involved in producing volatile aromatic compounds.en
dc.description.sponsorshipThis research was funded by Research Projects RTI2018-099371-B-I00 (MCIN/AEI/10.13039/ 5011000110333/ and FEDER Una manera de hacer Europa) and AGL2015-66833-R (MCIN/AEI/10.13039/ 5011000110333/ and FEDER Una manera de hacer Europa) of the Spanish National Research Programme, H2020 MUSA 727624 (EU), and by funds of the Public University of Navarre (UPNA). E.C.T. was supported by a grant of the Gobierno de Navarra (Ref. 0011-1408-2020-00000019) for Industrial Ph.D. students and by mobility grants from Erasmus+ program of Campus Iberus (CONS_2017-1- ES01-KA108-036811) and by a mobility grant of the Gobierno de Navarra, Spain.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.citationTomazeli, E. C., Alfaro, M., Zambonelli, A., Garde, E., Pérez, G., Jiménez, I., Ramírez, L., Salman, H., & Pisabarro, A. G. (2023). Transcriptome metabolic characterization of tuber borchii sp1—A new spanish strain for in vitro studies of the bianchetto truffle. International Journal of Molecular Sciences, 24(13), 10981. https://doi.org/10.3390/ijms241310981en
dc.identifier.doi10.3390/ijms241310981
dc.identifier.issn1661-6596
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/46385
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofInternational Journal of Molecular Sciences 2023, 24(13), 10981en
dc.relation.projectIDinfo:eu-repo/grantAgreement/European Commission/ERASMUS+/CONS_2017-1-ES01-KA108-036811/
dc.relation.projectIDinfo:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/727624/
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099371-B-I00/ES/
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2015-66833-R/ES/
dc.relation.projectIDinfo:eu-repo/grantAgreement/Gobierno de Navarra//0011-1408-2020-00000019/
dc.relation.publisherversionhttps://doi.org/10.3390/ijms241310981
dc.rights© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.es
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAxenic cultureen
dc.subjectAroma genesen
dc.subjectCentralmetabolismen
dc.subjectFermentationen
dc.subjectSecondarymetabolite clustersen
dc.subjectStrain isolationen
dc.subjectTrasncription efforten
dc.subjectTranscriptome profileen
dc.subjectTruffle cultivationen
dc.subjectVegetative myceliumen
dc.titleTranscriptome metabolic characterization of tuber borchii SP1-A new spanish strain for in vitro studies of the bianchetto truffleen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery21dc2f81-90e9-4ab4-bfb7-2b4260d2d72d

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