Quantitative proteomics reveals the importance of nitrogen source to control glucosinolate metabolism in Arabidopsis thaliana and Brassica oleracea

Marino Bilbao, Daniel; Ariz Arnedo, Idoia; Lasa Larrea, Berta; Santamaría Martínez, Enrique; Aparicio Tejo, Pedro María

Quantitative proteomics reveals the importance of nitrogen source to control glucosinolate metabolism in Arabidopsis thaliana and Brassica oleracea

dc.contributor.author	Marino Bilbao, Daniel
dc.contributor.author	Ariz Arnedo, Idoia
dc.contributor.author	Lasa Larrea, Berta
dc.contributor.author	Santamaría Martínez, Enrique
dc.contributor.author	Aparicio Tejo, Pedro María
dc.contributor.department	Ciencias del Medio Natural	es_ES
dc.contributor.department	Natura Ingurunearen Zientziak	eu
dc.date.accessioned	2020-09-22T07:53:18Z
dc.date.available	2020-09-22T07:53:18Z
dc.date.issued	2016
dc.description.abstract	Accessing different nitrogen (N) sources involves a profound adaptation of plant metabolism. In this study, a quantitative proteomic approach was used to further understand how the model plant Arabidopsis thaliana adjusts to different N sources when grown exclusively under nitrate or ammonium nutrition. Proteome data evidenced that glucosinolate metabolism was differentially regulated by the N source and that both TGG1 and TGG2 myrosinases were more abundant under ammonium nutrition, which is generally considered to be a stressful situation. Moreover, Arabidopsis plants displayed glucosinolate accumulation and induced myrosinase activity under ammonium nutrition. Interestingly, these results were also confirmed in the economically important crop broccoli (Brassica oleracea var. italica). Moreover, these metabolic changes were correlated in Arabidopsis with the differential expression of genes from the aliphatic glucosinolate metabolic pathway. This study underlines the importance of nitrogen nutrition and the potential of using ammonium as the N source in order to stimulate glucosinolate metabolism, which may have important applications not only in terms of reducing pesticide use, but also for increasing plants’ nutritional value.	en
dc.description.sponsorship	This research was financially supported by the Basque Government (IT932-16), the Spanish Ministry of Economy and Competitiveness (AGL2015-64582-C3-1-R and BIO2014-56271-R co-funded by FEDER), and the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007–2013) under REA grant agreement number 334019. Navarrabiomed Proteomics Unit is a member of ProteoRed, PRB2-ISCIII, and is supported by grant PT13/0001, of the PE I+D+I 2013-2016 funded by ISCIII and FEDER.	en
dc.format.extent	11 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1093/jxb/erw147
dc.identifier.issn	1460-2431
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/38217
dc.language.iso	eng	en
dc.publisher	Oxford University Press	en
dc.relation.ispartof	Journal of Experimental Botany, 2016, 67(11), 3313-3323	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//AGL2015-64582-C3-1-R/ES/
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//BIO2014-56271-R/ES/
dc.relation.projectID	info:eu-repo/grantAgreement/European Commission/FP7/334019/
dc.relation.publisherversion	https://doi.org/10.1093/jxb/erw147
dc.rights	© The Author 2016. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/3.0/
dc.subject	Ammonium	en
dc.subject	Arabidopsis thaliana	en
dc.subject	Broccoli	en
dc.subject	Glucosinolates	en
dc.subject	Myrosinase	en
dc.subject	Nitrate	en
dc.subject	Nitrogen nutrition	en
dc.subject	Proteomics	en
dc.title	Quantitative proteomics reveals the importance of nitrogen source to control glucosinolate metabolism in Arabidopsis thaliana and Brassica oleracea	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
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