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Strain degeneration in pleurotus ostreatus: a genotype dependent oxidative stress process which triggers oxidative stress, cellular detoxifying and cell wall reshaping genes

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dc.contributor.authorPérez Garrido, María Gumersinda
dc.contributor.authorLopez-Moya, Federico
dc.contributor.authorChuina Tomazeli, Emilia
dc.contributor.authorIbañez Vea, María
dc.contributor.authorGarde Sagardoy, Edurne
dc.contributor.authorLópez Llorca, Luis V.
dc.contributor.authorPisabarro de Lucas, Gerardo
dc.contributor.authorRamírez Nasto, Lucía
dc.contributor.departmentInstitute for Multidisciplinary Research in Applied Biology - IMABen
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes
dc.date.accessioned2022-04-12T10:43:57Z
dc.date.available2022-04-12T10:43:57Z
dc.date.issued2021
dc.description.abstractStrain degeneration has been defined as a decrease or loss in the yield of important commercial traits resulting from subsequent culture, which ultimately leads to Reactive Oxygen Species (ROS) production. Pleurotus ostreatus is a lignin-producing nematophagous edible mushroom. Mycelia for mushroom production are usually maintained in subsequent culture in solid media and frequently show symptoms of strain degeneration. The dikaryotic strain P. ostreatus (DkN001) has been used in our lab as a model organism for different purposes. Hence, different tools have been developed to uncover genetic and molecular aspects of this fungus. In this work, strain degeneration was studied in a full-sib monokaryotic progeny of the DkN001 strain with fast (F) and slow (S) growth rates by using different experimental approaches (light microscopy, malondialdehyde levels, whole-genome transcriptome analysis, and chitosan effect on monokaryotic mycelia). The results obtained showed that: (i) strain degeneration in P. ostreatus is linked to oxidative stress, (ii) the oxidative stress response in monokaryons is genotype dependent, (iii) stress and detoxifying genes are highly expressed in S monokaryons with symptoms of strain degeneration, (iv) chitosan addition to F and S monokaryons uncovered the constitutive expression of both oxidative stress and cellular detoxifying genes in S monokaryon strains which suggest their adaptation to oxidative stress, and (v) the overexpression of the cell wall genes, Uap1 and Cda1, in S monokaryons with strain degeneration phenotype indicates cell wall reshaping and the activation of High Osmolarity Glycerol (HOG) and Cell Wall Integrity (CWI) pathways. These results could constitute a hallmark for mushroom producers to distinguish strain degeneration in commercial mushrooms.en
dc.description.sponsorshipThis research was funded by Research Projects RTI2018-099371-B-I00 (MCIU, AEI, FEDER/UE) and AGL2015-66833-R (MINECO) of the Spanish National Research Programme, H2020 MUSA 727624 (EU), and by funds of the Public University of Navarre (UPNA).en
dc.format.extent26 p.
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.3390/jof7100862
dc.identifier.issn2309-608X
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/42746
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofJournal of Fungi, 7 (10), 2021en
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099371-B-I00/ES/en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2015-66833-R/ES/en
dc.relation.projectIDinfo:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/727624en
dc.relation.publisherversionhttps://doi.org/10.3390/jof7100862
dc.rights© 2021 by the authors. Creative Commons Attribution 4.0 Internationalen
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAlternative oxidaseen
dc.subjectChitosanen
dc.subjectEdible mushroomen
dc.subjectGrowth rateen
dc.subjectROS productionen
dc.titleStrain degeneration in pleurotus ostreatus: a genotype dependent oxidative stress process which triggers oxidative stress, cellular detoxifying and cell wall reshaping genesen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dspace.entity.typePublication
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relation.isAuthorOfPublication54daf1c4-f273-4ade-a90e-7df28e761ca7
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relation.isAuthorOfPublication.latestForDiscovery42c7e5c5-03c4-4a68-9f3f-06c1301753c0

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