Publication:
Staphylococcus aureus pathogenicity island DNA is packaged in particles composed of phage proteins

Date

2008

Authors

Tormo Más, María Ángeles
Ferrer, María Desamparados
Maiques, Elisa
Ubeda, Carles
Selva, Laura
Calvete, Juan J.
Novick, Richard P.
Penadés, José R.

Director

Publisher

American Society for Microbiology
Acceso abierto / Sarbide irekia
Artículo / Artikulua
Versión publicada / Argitaratu den bertsioa

Project identifier

Abstract

Staphylococcus aureus pathogenicity islands (SaPIs) have an intimate relationship with temperate staphylococcal phages. During phage growth, SaPIs are induced to replicate and are efficiently encapsidated into special small phage heads commensurate with their size. We have analyzed by amino acid sequencing and mass spectrometry the protein composition of the specific SaPI particles. This has enabled identification of major capsid and tail proteins and a putative portal protein. As expected, all these proteins were phage encoded. Additionally, these analyses suggested the existence of a protein required for the formation of functional phage but not SaPI particles. Mutational analysis demonstrated that the phage proteins identified were involved only in the formation and possibly the function of SaPI or phage particles, having no role in other SaPI or phage functions.

Description

Keywords

Staphylococcus aureus, Staphylococcus Phages, Viral proteins, Genetics

Department

IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua

Faculty/School

Degree

Doctorate program

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© 2008, American Society for Microbiology. All Rights Reserved.

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