Publication:
Fermentation and alternative oxidase contribute to the action of amino acid biosynthesis-inhibiting herbicides

Date

2015

Director

Publisher

Elsevier
Acceso abierto / Sarbide irekia
Artículo / Artikulua
Versión aceptada / Onetsi den bertsioa

Project identifier

MICINN//AGL2010-18621/ES/recolecta
MINECO//AGL2013-40567-R/ES/recolecta

Abstract

Acetolactate synthase inhibitors (ALS-inhibitors) and glyphosate (GLP) are two classes of herbicide that act by the specific inhibition of an enzyme in the biosynthetic pathway of branched-chain or aromatic amino acids, respectively. The physiological effects that are detected after application of these two classes of herbicides are not fully understood in relation to the primary biochemical target inhibition, although they have been well documented. Interestingly, the two herbicides’ toxicity includes some common physiological effects suggesting that they kill the treated plants by a similar pattern despite targeting different enzymes. The induction of aerobic ethanol fermentation and alternative oxidase (AOX) are two examples of these common effects. The objective of this work was to gain further insight into the role of fermentation and AOX induction in the toxic consequences of ALS-inhibitors and GLP. For this, Arabidopsis T-DNA knockout mutants of alcohol dehydrogenase (ADH) 1 and AOX1a were used. The results found in wild-type indicate that both GLP and ALS-inhibitors reduce ATP production by inducing fermentation and alternative respiration. The main physiological effects in the process of herbicide activity upon treated plants were accumulation of carbohydrates and total free amino acids. The effects of the herbicides on these parameters were less pronounced in mutants compared to wild-type plants. The role of fermentation and AOX regarding pyruvate availability is also discussed.

Description

Incluye 1 fichero de datos

Keywords

Glyphosate, Acetolactate synthase inhibitors, Ethanol fermentation, Arabidopsis thaliana, Physiological effects

Department

Ciencias del Medio Natural / Natura Ingurunearen Zientziak

Faculty/School

Degree

Doctorate program

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© 2014 Elsevier GmbH. The manuscript version is made available under the CC BY-NC-ND 4.0 license.

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