Fermentation and alternative oxidase contribute to the action of amino acid biosynthesis-inhibiting herbicides

Zulet González, Amaia; Gil Monreal, Miriam; Zabalza Aznárez, Ana; Dongen, Joost T. van; Royuela Hernando, Mercedes

Publication:
Fermentation and alternative oxidase contribute to the action of amino acid biosynthesis-inhibiting herbicides

dc.contributor.author	Zulet González, Amaia
dc.contributor.author	Gil Monreal, Miriam
dc.contributor.author	Zabalza Aznárez, Ana
dc.contributor.author	Dongen, Joost T. van	es_ES
dc.contributor.author	Royuela Hernando, Mercedes
dc.contributor.department	Ciencias del Medio Natural	es_ES
dc.contributor.department	Natura Ingurunearen Zientziak	eu
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2017-04-11T16:40:42Z
dc.date.available	2017-04-11T16:40:42Z
dc.date.issued	2015
dc.description	Incluye 1 fichero de datos	es_ES
dc.description.abstract	Acetolactate synthase inhibitors (ALS-inhibitors) and glyphosate (GLP) are two classes of herbicide that act by the specific inhibition of an enzyme in the biosynthetic pathway of branched-chain or aromatic amino acids, respectively. The physiological effects that are detected after application of these two classes of herbicides are not fully understood in relation to the primary biochemical target inhibition, although they have been well documented. Interestingly, the two herbicides’ toxicity includes some common physiological effects suggesting that they kill the treated plants by a similar pattern despite targeting different enzymes. The induction of aerobic ethanol fermentation and alternative oxidase (AOX) are two examples of these common effects. The objective of this work was to gain further insight into the role of fermentation and AOX induction in the toxic consequences of ALS-inhibitors and GLP. For this, Arabidopsis T-DNA knockout mutants of alcohol dehydrogenase (ADH) 1 and AOX1a were used. The results found in wild-type indicate that both GLP and ALS-inhibitors reduce ATP production by inducing fermentation and alternative respiration. The main physiological effects in the process of herbicide activity upon treated plants were accumulation of carbohydrates and total free amino acids. The effects of the herbicides on these parameters were less pronounced in mutants compared to wild-type plants. The role of fermentation and AOX regarding pyruvate availability is also discussed.	en
dc.description.sponsorship	This work was financially supported through a grant from the Ministerio Espanol de Ciencia y Tecnología (AGL-2010-18621 and AGL-2013-40567-R). A. Zulet and M. Gil-Monreal received funding from fellowships through the Ministerio de Educación and the Universidad Pública de Navarra, respectively.	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1016/j.jplph.2014.12.004
dc.identifier.issn	0176-1617 (Print)
dc.identifier.issn	1618-1328 (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/24092
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	Journal of Plant Physiology 175 (2015) 102–112	en
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//AGL2010-18621/ES/	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//AGL2013-40567-R/ES/	en
dc.relation.publisherversion	http://doi.org/10.1016/j.jplph.2014.12.004
dc.rights	© 2014 Elsevier GmbH. The manuscript version is made available under the CC BY-NC-ND 4.0 license.	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Glyphosate	en
dc.subject	Acetolactate synthase inhibitors	en
dc.subject	Ethanol fermentation	en
dc.subject	Arabidopsis thaliana	en
dc.subject	Physiological effects	en
dc.title	Fermentation and alternative oxidase contribute to the action of amino acid biosynthesis-inhibiting herbicides	en
dc.type	Artículo / Artikulua	es
dc.type	info:eu-repo/semantics/article	en
dc.type.version	Versión aceptada / Onetsi den bertsioa	es
dc.type.version	info:eu-repo/semantics/acceptedVersion	en
dspace.entity.type	Publication
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