Publication: Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
| dc.contributor.author | Ancín Rípodas, María | |
| dc.contributor.author | Larraya Reta, Luis María | |
| dc.contributor.author | Florez-Sarasa, Igor | |
| dc.contributor.author | Bénard, Camille | |
| dc.contributor.author | Fernández San Millán, Alicia | |
| dc.contributor.author | Veramendi Charola, Jon | |
| dc.contributor.author | Gibon, Yves | |
| dc.contributor.author | Fernie, Alisdair R. | |
| dc.contributor.author | Aranjuelo Michelena, Iker | |
| dc.contributor.author | Farrán Blanch, Inmaculada | |
| dc.contributor.department | Agronomia, Bioteknologia eta Elikadura | eu |
| dc.contributor.department | Institute for Multidisciplinary Research in Applied Biology - IMAB | en |
| dc.contributor.department | Agronomía, Biotecnología y Alimentación | es_ES |
| dc.date.accessioned | 2021-12-30T06:39:55Z | |
| dc.date.available | 2021-12-30T06:39:55Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts. | en |
| dc.description.sponsorship | This work was supported by the Spanish Ministry of Science and Innovation (AGL2016-79868) and Phenome-FPPN (ANR-11-INBS-0012). MA is a recipient of a PhD fellowship from the Spanish Ministry of Education (FPU13/01675). IFS has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 753301, the ‘Ramon y Cajal’ contract RYC2019-027244-I/AEI/10.13039/501100011033, and the European Social Fund. | en |
| dc.format.extent | 16 p. | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | 10.1093/jxb/erab193 | |
| dc.identifier.issn | 0022-0957 | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/41656 | |
| dc.language.iso | eng | en |
| dc.publisher | Oxford University Press | en |
| dc.relation.ispartof | Journal of Experimental Botany, 72 (13), 4949-4964 | en |
| dc.relation.projectID | info:eu-repo/grantAgreement/ES/1PE/AGL2016-79868 | en |
| dc.relation.projectID | info:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/753301 | en |
| dc.relation.publisherversion | http://doi.org/10.1093/jxb/erab193 | |
| dc.rights | © The Author(s) 2021. Creative Commons Attribution 4.0 International | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Carbon metabolism | en |
| dc.subject | Chloroplast | en |
| dc.subject | Glutamine synthetase | en |
| dc.subject | GS-GOGAT pathway | en |
| dc.subject | Nitrogen metabolism | en |
| dc.subject | Photorespiration | en |
| dc.subject | Thioredoxin | en |
| dc.title | Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | en |
| dc.type.version | Versión publicada / Argitaratu den bertsioa | es |
| dspace.entity.type | Publication | |
| relation.isAuthorOfPublication | 7ac62eb7-ab79-45a4-b8ba-3ecbd92c4e64 | |
| relation.isAuthorOfPublication | 62d7811e-d4cb-42e6-90ee-8e9dc4f8a95c | |
| relation.isAuthorOfPublication | 9c84c660-8e77-4487-bd7a-07ad058b0425 | |
| relation.isAuthorOfPublication | a901e048-1a47-49df-a6f4-c2012c88c29e | |
| relation.isAuthorOfPublication | b8dd84ae-83ed-4e3f-873e-b0023505b3df | |
| relation.isAuthorOfPublication | 6442ab25-7b61-4611-a7b8-d237c8cf5d82 | |
| relation.isAuthorOfPublication.latestForDiscovery | 7ac62eb7-ab79-45a4-b8ba-3ecbd92c4e64 |