Publication:
Expression of key myogenic, fibrogenic and adipogenic genes in Longissimus thoracis and masseter muscles in cattle

dc.contributor.authorMartínez del Pino, Lara
dc.contributor.authorUrrutia Vera, Olaia
dc.contributor.authorArana Navarro, Ana
dc.contributor.authorAlfonso Ruiz, Leopoldo
dc.contributor.authorMendizábal Aizpuru, José Antonio
dc.contributor.authorSoret Lafraya, Beatriz
dc.contributor.departmentAgronomia, Bioteknologia eta Elikaduraeu
dc.contributor.departmentInstitute on Innovation and Sustainable Development in Food Chain - ISFOODen
dc.contributor.departmentAgronomía, Biotecnología y Alimentaciónes_ES
dc.date.accessioned2020-06-05T10:28:33Z
dc.date.available2020-07-20T23:00:13Z
dc.date.issued2020
dc.description.abstractAdipogenesis, myogenesis and fibrogenesis are related processes that can contribute to meat quality. Therefore, extending the knowledge of these processes would facilitate the identification of molecular markers that predict intramuscular fat accretion. The main purpose of this work, based on previous results, was to further study the expression of key genes related to adipogenic, myogenic, fibrogenic processes and some cytokines in Longissimus thoracis (LT) and Masseter (MS) muscles of Pirenaica and Holstein young bulls. Longissimus thoracis and MS muscles from Pirenaica (n = 4) and Spanish Holstein (n = 4) were sampled for proximate analysis, determination of adipocyte size distribution and expression of key candidate genes. Fat percentage was lower in LT than in MS muscle in Pirenaica young bulls (P = 0.023) and was higher in LT muscle in Holstein than in Pirenaica young bulls (P = 0.007). Gene expression analysis revealed that the mRNA level of myogenic differentiation 1 (MYOD) was higher in LT than in MS muscles in both groups of animals (P < 0.001) and that myostatin (MSTN) expression was also higher in LT than in MS muscle in Holstein bulls (P = 0.001). On the other hand, MSTN and PPARG showed higher expression in LT and MS in Pirenaica young bulls (P = 0.026), while the expression of fatty acid-binding protein 4 (FABP4) was higher in Holstein young bulls, also in both muscles (P < 0.001). The results suggested that the development of intramuscular adipose depot was directly related to the expression of adipogenic genes, such as FABP4, but inversely related to the expression of the cytokine MSTN and the myogenic gene MYOD, genes which showed a muscle-specific expression.en
dc.description.sponsorshipThe work was partially supported by Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria grant number ‘RTA2013-00046-C03-03’ (JAM and AA).en
dc.embargo.lift2020-07-20
dc.embargo.terms2020-07-20
dc.format.extent32 p.
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.1017/S1751731120000051
dc.identifier.issn1751-7311
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/37093
dc.language.isoengen
dc.publisherCambridge University Pressen
dc.relation.ispartofAnimal, 2020, 1-10en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//RTA2013-00046-C03-03/ES/en
dc.relation.publisherversionhttps://doi.org/10.1017/S1751731120000051
dc.rights© The Animal Consortium 2020en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.subjectMarblingen
dc.subjectAdipogenesisen
dc.subjectMyogenesisen
dc.subjectCytokinesen
dc.subjectTranscriptomeen
dc.titleExpression of key myogenic, fibrogenic and adipogenic genes in Longissimus thoracis and masseter muscles in cattleen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/acceptedVersionen
dc.type.versionVersión aceptada / Onetsi den bertsioaes
dspace.entity.typePublication
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relation.isAuthorOfPublicationee903831-0350-48ee-9595-de9942e3a89b
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relation.isAuthorOfPublication.latestForDiscovery453b1ddd-a6b8-4863-9a83-0a47f6eda88a

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