Publication:
Endophytic fungi from kale (Brassica oleracea var. acephala) modify roots-glucosinolate profile and promote plant growth in cultivated Brassica species. First description of Pyrenophora gallaeciana

Date

2022

Authors

Rodríguez, Víctor Manuel
Díaz Urbano, María
Sklenář, František
Saati-Santamaría, Zaki
Menéndez, Esther
Velasco, Pablo

Director

Publisher

Frontiers Media
Acceso abierto / Sarbide irekia
Artículo / Artikulua
Versión publicada / Argitaratu den bertsioa

Project identifier

European Commission/Horizon 2020 Framework Programme/897795openaire
AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096591-B-I00/ES/recolecta

Abstract

Endophytic fungi of crops can promote plant growth through various mechanisms of action (i.e., improve nutrient uptake and nutrient use efficiency, and produce and modulate plant hormones). The genus Brassica includes important horticultural crops, which have been little studied in their interaction with endophytic fungi. Previously, four endophytic fungi were isolated from kale roots (Brassica oleracea var. acephala), with different benefits for their host, including plant growth promotion, cold tolerance, and induction of resistance to pathogens (Xanthomonas campestris) and pests (Mamestra brassicae). In the present work, the molecular and morphological identification of the four different isolates were carried out, describing them as the species Acrocalymma vagum, Setophoma terrestris, Fusarium oxysporum, and the new species Pyrenophora gallaeciana. In addition, using a representative crop of each Brassica U’s triangle species and various in vitro biochemical tests, the ability of these fungi to promote plant growth was described. In this sense, the four fungi used promoted the growth of B. rapa, B. napus, B. nigra, B. juncea, and B. carinata, possibly due to the production of auxins, siderophores, P solubilization or cellulase, xylanase or amylase activity. Finally, the differences in root colonization between the four endophytic fungi and two pathogens (Leptosphaeria maculans and Sclerotinia sclerotiorum) and the root glucosinolate profile were studied, at different times. In this way, how the presence of progoitrin in the roots reduces their colonization by endophytic and pathogenic fungi was determined, while the possible hydrolysis of sinigrin to fungicidal products controls the colonization of endophytic fungi, but not of pathogens.

Description

Keywords

Acrocalymma vagum, Brassica U's triangle, Fusarium oxysporum, Glucosinolates, Indoleacetic acid, Setophoma terrestris, Sinigrin

Department

Institute for Multidisciplinary Research in Applied Biology - IMAB

Faculty/School

Degree

Doctorate program

item.page.cita

Poveda, J., Rodríguez, V. M., Díaz-Urbano, M., Sklenář, F., Saati-Santamaría, Z., Menéndez, E., & Velasco, P. (2022). Endophytic fungi from kale (Brassica oleracea var. Acephala) modify roots-glucosinolate profile and promote plant growth in cultivated Brassica species. First description of Pyrenophora gallaeciana. Frontiers in Microbiology, 13, 981507. https://doi.org/10.3389/fmicb.2022.981507

item.page.rights

© 2022 Poveda, Rodríguez, Díaz-Urbano, Sklenář, Saati-Santamaría, Menéndez and Velasco. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).

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