Endophytic fungi from kale (Brassica oleracea var. acephala) modify roots-glucosinolate profile and promote plant growth in cultivated Brassica species. First description of Pyrenophora gallaeciana

Poveda Arias, Jorge; Rodríguez, Víctor Manuel; Díaz Urbano, María; Sklenář, František; Saati-Santamaría, Zaki; Menéndez, Esther; Velasco, Pablo

Publication:
Endophytic fungi from kale (Brassica oleracea var. acephala) modify roots-glucosinolate profile and promote plant growth in cultivated Brassica species. First description of Pyrenophora gallaeciana

dc.contributor.author	Poveda Arias, Jorge
dc.contributor.author	Rodríguez, Víctor Manuel
dc.contributor.author	Díaz Urbano, María
dc.contributor.author	Sklenář, František
dc.contributor.author	Saati-Santamaría, Zaki
dc.contributor.author	Menéndez, Esther
dc.contributor.author	Velasco, Pablo
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.date.accessioned	2023-04-26T11:41:21Z
dc.date.available	2023-04-26T11:41:21Z
dc.date.issued	2022
dc.date.updated	2023-04-26T11:18:03Z
dc.description.abstract	Endophytic fungi of crops can promote plant growth through various mechanisms of action (i.e., improve nutrient uptake and nutrient use efficiency, and produce and modulate plant hormones). The genus Brassica includes important horticultural crops, which have been little studied in their interaction with endophytic fungi. Previously, four endophytic fungi were isolated from kale roots (Brassica oleracea var. acephala), with different benefits for their host, including plant growth promotion, cold tolerance, and induction of resistance to pathogens (Xanthomonas campestris) and pests (Mamestra brassicae). In the present work, the molecular and morphological identification of the four different isolates were carried out, describing them as the species Acrocalymma vagum, Setophoma terrestris, Fusarium oxysporum, and the new species Pyrenophora gallaeciana. In addition, using a representative crop of each Brassica U’s triangle species and various in vitro biochemical tests, the ability of these fungi to promote plant growth was described. In this sense, the four fungi used promoted the growth of B. rapa, B. napus, B. nigra, B. juncea, and B. carinata, possibly due to the production of auxins, siderophores, P solubilization or cellulase, xylanase or amylase activity. Finally, the differences in root colonization between the four endophytic fungi and two pathogens (Leptosphaeria maculans and Sclerotinia sclerotiorum) and the root glucosinolate profile were studied, at different times. In this way, how the presence of progoitrin in the roots reduces their colonization by endophytic and pathogenic fungi was determined, while the possible hydrolysis of sinigrin to fungicidal products controls the colonization of endophytic fungi, but not of pathogens.	en
dc.description.sponsorship	This research was financially supported by projects RTI2018-096591-B-I00 34 (MCIU/AEI/FEDER, UE) and IN607A 2021/03 (Xunta de Galicia, Spain). EM acknowledges an European Union’s Horizon 2020 Marie Sklodowska-Curie Actions (Grant Agreement nº 897795).	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.citation	Poveda, J., Rodríguez, V. M., Díaz-Urbano, M., Sklenář, F., Saati-Santamaría, Z., Menéndez, E., & Velasco, P. (2022). Endophytic fungi from kale (Brassica oleracea var. Acephala) modify roots-glucosinolate profile and promote plant growth in cultivated Brassica species. First description of Pyrenophora gallaeciana. Frontiers in Microbiology, 13, 981507. https://doi.org/10.3389/fmicb.2022.981507	en
dc.identifier.doi	10.3389/fmicb.2022.981507
dc.identifier.issn	1664-302X
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/45198
dc.language.iso	eng	en
dc.publisher	Frontiers Media	en
dc.relation.ispartof	Frontiers in Microbiology, 2022, 13, 1-14	en
dc.relation.projectID	info:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/897795	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096591-B-I00/ES/	en
dc.relation.publisherversion	https://doi.org/10.3389/fmicb.2022.981507
dc.rights	© 2022 Poveda, Rodríguez, Díaz-Urbano, Sklenář, Saati-Santamaría, Menéndez and Velasco. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	Acrocalymma vagum	en
dc.subject	Brassica U's triangle	en
dc.subject	Fusarium oxysporum	en
dc.subject	Glucosinolates	en
dc.subject	Indoleacetic acid	en
dc.subject	Setophoma terrestris	en
dc.subject	Sinigrin	en
dc.title	Endophytic fungi from kale (Brassica oleracea var. acephala) modify roots-glucosinolate profile and promote plant growth in cultivated Brassica species. First description of Pyrenophora gallaeciana	en
dc.type	info:eu-repo/semantics/article
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dspace.entity.type	Publication
relation.isAuthorOfPublication	85d07390-8e9d-444e-a969-87fff3f1497e
relation.isAuthorOfPublication.latestForDiscovery	85d07390-8e9d-444e-a969-87fff3f1497e