Phylogenetic analysis of the pPT23A plasmid family of Pseudomonas syringae
Fecha
2006Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1128/aem.01923-06
Resumen
The pPT23A plasmid family of Pseudomonas syringae contains members that contribute to the ecological and
pathogenic fitness of their P. syringae hosts. In an effort to understand the evolution of these plasmids and their
hosts, we undertook a comparative analysis of the phylogeny of plasmid genes and that of conserved chromosomal
genes from P. syringae. In total, comparative sequence and phylogen ...
[++]
The pPT23A plasmid family of Pseudomonas syringae contains members that contribute to the ecological and
pathogenic fitness of their P. syringae hosts. In an effort to understand the evolution of these plasmids and their
hosts, we undertook a comparative analysis of the phylogeny of plasmid genes and that of conserved chromosomal
genes from P. syringae. In total, comparative sequence and phylogenetic analyses were done utilizing 47
pPT23A family plasmids (PFPs) from 16 pathovars belonging to six genomospecies. Our results showed that
the plasmid replication gene (repA), the only gene currently known to be distributed among all the PFPs, had
a phylogeny that was distinct from that of the P. syringae hosts of these plasmids and from those of other
individual genes on PFPs. The phylogenies of two housekeeping chromosomal genes, those for DNA gyrase B
subunit (gyrB) and primary sigma factor (rpoD), however, were strongly associated with genomospecies of P.
syringae. Based on the results from this study, we conclude that the pPT23A plasmid family represents a
dynamic genome that is mobile among P. syringae pathovars. [--]
Materias
Pseudomonas syringae,
pPT23A
Editor
American Society for Microbiology
Publicado en
Applied and Environmental Microbiology, Feb. 2007, Vol. 73, No. 4, p. 1287–1295
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
Versión del editor
Entidades Financiadoras
This work was supported by the following agencies: the United
States Department of Agriculture (USDA-NRI 2003-01531 to G.W.S.)
and the Michigan Agricultural Experiment Station (Z.M., J.J.S., Y.Z.,
and G.W.S.), the UK Biotechnology and Biological Sciences Research
Council (R.W.J. and D.L.A.), and the Spanish Ministerio de Educación y Ciencia (AGL2004-03143 [J.M.]).