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dc.creatorCucarella, Carmees_ES
dc.creatorTormo Más, María Ángeleses_ES
dc.creatorUbeda, Carleses_ES
dc.creatorTrotonda, María Pilares_ES
dc.creatorMonzon, Martaes_ES
dc.creatorPeris, Cristòfoles_ES
dc.creatorAmorena Zabalza, Beatrizes_ES
dc.creatorLasa Uzcudun, Íñigoes_ES
dc.creatorPenadés, José R.es_ES
dc.date.accessioned2019-01-23T11:16:20Z
dc.date.available2019-01-23T11:16:20Z
dc.date.issued2004
dc.identifier.issn0019-9567 (Print)
dc.identifier.issn1098-5522 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/32083
dc.description.abstractStaphylococcus aureus is a common cause of intramammary infections, which frequently become chronic, associated with the ability of the bacteria to produce biofilm. Here, we report a relationship between the ability to produce chronic bovine mastitis and biofilm formation. We have classified bovine mastitis S. aureus isolates into three groups based on the presence of particular genetic elements required for biofilm formation: group 1 (ica+ bap+), group 2 (ica+, bap negative), and group 3 (ica negative, bap negative). Overall, animals naturally infected with group 1 and 2 isolates had a lower milk somatic cell count than those infected with isolates of group 3. In addition, Bap-positive isolates were significantly more able to colonize and persist in the bovine mammary gland in vivo and were less susceptible to antibiotic treatments when forming biofilms in vitro. Analysis of the structural bap gene revealed the existence of alternate forms of expression of the Bap protein in S. aureus isolates obtained under field conditions throughout the animal's life. The presence of anti-Bap antibodies in serum samples taken from animals with confirmed S. aureus infections indicated the production of Bap during infection. Furthermore, disruption of the ica operon in a bap-positive strain had no effect on in vitro biofilm formation, a finding which strongly suggested that Bap could compensate for the deficiency of the PIA/PNAG product (a biofilm matrix polysaccharide). Altogether, these results demonstrate that, in the bovine intramammary gland, the presence of Bap may facilitate a biofilm formation connected with the persistence of S. aureus.en
dc.description.sponsorshipThis work was supported by grants BIO99-0285 and BIO2002-04542-C02-01 from the Comisión Interministerial de Ciencia y Tecnología (C.I.C.Y.T.) and grants from the Cardenal Herrera-CEU University and Conselleria de Agricultura, Pesca i Alimentació (Generalitat Valenciana) to J.R.P. Fellowship support for Carme Cucarella, María Ángeles Tormo, and Pilar Trotonda from the Cardenal Herrera-CEU University and for Carles Úbeda from the Conselleria de Agricultura, Pesca i Alimentació (Generalitat Valenciana) is gratefully acknowledged.en
dc.format.extent9 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofInfection and immunity, vol. 72, nº 4, apr. 2004, p. 2177–2185en
dc.rights© 2004, American Society for Microbiology. All Rights Reserved.en
dc.subjectBiofilmsen
dc.subjectStaphylococcus aureusen
dc.subjectBacterial proteinsen
dc.subjectInfectionsen
dc.titleRole of biofilm-associated protein bap in the pathogenesis of bovine Staphylococcus aureusen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentIdAB – Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/iai.72.4.2177-2185.2004
dc.relation.publisherversionhttps://doi.org/10.1128/iai.72.4.2177-2185.2004
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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