The enterococcal surface protein, Esp, is involved in Enterococcus faecalis biofilm formation
Fecha
2001Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1128/aem.67.10.4538-4545.2001
Resumen
The enterococcal surface protein, Esp, is a high-molecular-weight surface protein of unknown function whose
frequency is significantly increased among infection-derived Enterococcus faecalis isolates. In this work, a global
structural similarity was found between Bap, a biofilm-associated protein of Staphylococcus aureus, and Esp.
Analysis of the relationship between the presence of the Esp-encod ...
[++]
The enterococcal surface protein, Esp, is a high-molecular-weight surface protein of unknown function whose
frequency is significantly increased among infection-derived Enterococcus faecalis isolates. In this work, a global
structural similarity was found between Bap, a biofilm-associated protein of Staphylococcus aureus, and Esp.
Analysis of the relationship between the presence of the Esp-encoding gene (esp) and the biofilm formation
capacity in E. faecalis demonstrated that the presence of the esp gene is highly associated (P < 0.0001) with the
capacity of E. faecalis to form a biofilm on a polystyrene surface, since 93.5% of the E. faecalis esp-positive
isolates were capable of forming a biofilm. Moreover, none of the E. faecalis esp-deficient isolates were biofilm
producers. Depending on the E. faecalis isolate, insertional mutagenesis of esp caused either a complete loss of
the biofilm formation phenotype or no apparent phenotypic defect. Complementation studies revealed that Esp
expression in an E. faecalis esp-deficient strain promoted primary attachment and biofilm formation on
polystyrene and polyvinyl chloride plastic from urine collection bags. Together, these results demonstrate that
(i) biofilm formation capacity is widespread among clinical E. faecalis isolates, (ii) the biofilm formation
capacity is restricted to the E. faecalis strains harboring esp, and (iii) Esp promotes primary attachment and
biofilm formation of E. faecalis on abiotic surfaces. [--]
Materias
Enterococcal surface protein,
Enterococcus faecalis
Editor
American Society for Microbiology
Publicado en
Applied and Environmental Microbiology, Oct. 2001, Vol. 67, No. 10, p. 4538–4545
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This work was supported by grant BIO99-0285 from the Comisión
Interministerial de Ciencia y Tecnología and grants from the Cardenal
Herrera-CEU University and from the Departamento de Educación y
Cultura del Gobierno de Navarra.