Mostrar el registro sencillo del ítem
Near-infrared fluorescence imaging as an alternative to bioluminescent bacteria to monitor biomaterial-associated infections
dc.creator | Dinjaski, Nina | es_ES |
dc.creator | Shalu, Suri | es_ES |
dc.creator | Valle Turrillas, Jaione | es_ES |
dc.creator | Lehman, Susan M. | es_ES |
dc.creator | Lasa Uzcudun, Íñigo | es_ES |
dc.creator | Prieto, María Auxiliadora | es_ES |
dc.creator | García, Andrés J. | es_ES |
dc.date.accessioned | 2019-04-03T07:22:22Z | |
dc.date.available | 2019-04-03T07:22:22Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1742-7061 | |
dc.identifier.uri | https://hdl.handle.net/2454/32789 | |
dc.description.abstract | Biomaterial-associated infection is one of the most common complications related with the implantation of any biomedical device. Several in vivo imaging platforms have emerged as powerful diagnostic tools to longitudinally monitor biomaterial-associated infections in small animal models. In this study, we directly compared two imaging approaches: bacteria engineered to produce luciferase to generate bioluminescence and reactive oxygen species (ROS) imaging of the inflammatory response associated with the infected implant. We performed longitudinal imaging of bioluminescence associated with bacteria strains expressing plasmid-integrated luciferase driven by different promoters or a strain with the luciferase gene integrated into the chromosome. These luminescent strains provided adequate signal for acute (0–4 days) monitoring of the infection, but the bioluminescence signal decreased over time and leveled off by 7 days post-implantation. This loss in bioluminescence signal was attributed to changes in the metabolic activity of the bacteria. In contrast, near-infrared fluorescence imaging of ROS associated with inflammation to the implant provided sensitive and dose-dependent signals of biomaterialassociated bacteria. ROS imaging exhibited higher sensitivity than the bioluminescence imaging and was independent of the bacteria strain. Near-infrared fluorescence imaging of inflammatory responses represents a powerful alternative to bioluminescence imaging for monitoring biomaterial-associated bacterial infections. | en |
dc.description.sponsorship | This work was supported by the Ministerio of Economía y Competitividad (BIO2010-21049, 201120E092), the U.S.A. National Institutes of Health grant R21 AI094624 (A.J.G.), the Georgia Tech/Emory Center for the Engineering of Living Tissues, the Atlanta Clinical and Translational Science Institute under PHS Grant UL RR025008 from the Clinical and Translational Science Award Program. | en |
dc.format.extent | 18 p. | |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | Elsevier | en |
dc.relation.ispartof | Acta Biomaterialia 10 (2014) 2935-2944 | en |
dc.rights | © 2014 Acta Materialia Inc. Published by Elsevier Ltd. The manuscript version is made available under the CC BY-NC-ND 4.0 license | en |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | Biomaterial-associated infection | en |
dc.subject | Bioluminescence | en |
dc.subject | Near infrared fluorescence | en |
dc.subject | Noninvasive monitoring | en |
dc.subject | Staphylococcus aureus | en |
dc.title | Near-infrared fluorescence imaging as an alternative to bioluminescent bacteria to monitor biomaterial-associated infections | en |
dc.type | info:eu-repo/semantics/article | en |
dc.type | Artículo / Artikulua | es |
dc.contributor.department | IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
dc.identifier.doi | 10.1016/j.actbio.2014.03.005 | |
dc.relation.publisherversion | https://doi.org/10.1016/j.actbio.2014.03.005 | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | en |
dc.type.version | Versión aceptada / Onetsi den bertsioa | es |