The mangotoxin biosynthetic operon (mbo) is specifically distributed within Pseudomonas syringae genomospecies 1 and was acquired only once during evolution
Fecha
2013Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Identificador del proyecto
ES/6PN/AGL2008-55311
Impacto
|
10.1128/AEM.03007-12
Resumen
Mangotoxin production was first described in Pseudomonas syringae pv. syringae strains. A phenotypic characterization of 94 P. syringae strains was carried out to determine the genetic evolution of the mangotoxin biosynthetic operon (mbo). We designed a PCR primer pair specific for the mbo operon to examine its distribution within the P. syringae complex. These primers amplified a 692-bp DNA fragmen ...
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Mangotoxin production was first described in Pseudomonas syringae pv. syringae strains. A phenotypic characterization of 94 P. syringae strains was carried out to determine the genetic evolution of the mangotoxin biosynthetic operon (mbo). We designed a PCR primer pair specific for the mbo operon to examine its distribution within the P. syringae complex. These primers amplified a 692-bp DNA fragment from 52 mangotoxin-producing strains and from 7 non-mangotoxin-producing strains that harbor the mbo operon, whereas 35 non-mangotoxin-producing strains did not yield any amplification. This, together with the analysis of draft genomes, allowed the identification of the mbo operon in five pathovars (pathovars aptata, avellanae, japonica, pisi, and syringae), all of which belong to genomospecies 1, suggesting a limited distribution of the mbo genes in the P. syringae complex. Phylogenetic analyses using partial sequences from housekeeping genes differentiated three groups within genomospecies 1. All of the strains containing the mbo operon clustered in groups I and II, whereas those lacking the operon clustered in group III;
however, the relative branching order of these three groups is dependent on the genes used to construct the phylogeny. The mbo operon maintains synteny and is inserted in the same genomic location, with high sequence conservation around the insertion point, for all the strains in groups I and II. These data support the idea that the mbo operon was acquired horizontally and only once by the ancestor of groups I and II from genomospecies 1 within the P. syringae complex. [--]
Materias
Mangotoxin,
Phylogeny,
Diagnostic PCR,
Antimetabolite toxins,
Virulence,
Mango apical necrosis,
Lateral gene transfer
Editor
American Society for Microbiology
Publicado en
Applied and Environmental Microbiology 2013, 79 (3)
Notas
Incluye un fichero de datos
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
Versión del editor
Entidades Financiadoras
This work was supported by grants from the regional government of Andalucía
(Spain), grants from CICE-Junta de Andalucía, Ayudas Grupo
PAIDI AGR-169, and Proyecto de Excelencia (P07-AGR-02471) and Plan
Nacional IDI grant AGL2008-55311-CO2-01 (Ministerio de Ciencia e
Innovación), cofinanced by FEDER (European Union). Plan Propio of
the University of Málaga funded a stay by V.J.C. at the Universidad
Pública de Navarra, Spain.