Show simple item record

dc.creatorCarrión, Víctor J.es_ES
dc.creatorGutiérrez Barranquero, José Antonioes_ES
dc.creatorArrebola, Evaes_ES
dc.creatorBardají Goikoetxea, Leirees_ES
dc.creatorCodina, Juan Carloses_ES
dc.creatorVicente, Antonio dees_ES
dc.creatorCazorla, Francisco M.es_ES
dc.creatorMurillo Martínez, Jesúses_ES
dc.date.accessioned2017-03-15T18:00:12Z
dc.date.available2017-03-15T18:00:12Z
dc.date.issued2013
dc.identifier.issn0099-2240 (Print)
dc.identifier.issn1098-5336 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/23909
dc.descriptionIncluye un fichero de datos
dc.description.abstractMangotoxin production was first described in Pseudomonas syringae pv. syringae strains. A phenotypic characterization of 94 P. syringae strains was carried out to determine the genetic evolution of the mangotoxin biosynthetic operon (mbo). We designed a PCR primer pair specific for the mbo operon to examine its distribution within the P. syringae complex. These primers amplified a 692-bp DNA fragment from 52 mangotoxin-producing strains and from 7 non-mangotoxin-producing strains that harbor the mbo operon, whereas 35 non-mangotoxin-producing strains did not yield any amplification. This, together with the analysis of draft genomes, allowed the identification of the mbo operon in five pathovars (pathovars aptata, avellanae, japonica, pisi, and syringae), all of which belong to genomospecies 1, suggesting a limited distribution of the mbo genes in the P. syringae complex. Phylogenetic analyses using partial sequences from housekeeping genes differentiated three groups within genomospecies 1. All of the strains containing the mbo operon clustered in groups I and II, whereas those lacking the operon clustered in group III; however, the relative branching order of these three groups is dependent on the genes used to construct the phylogeny. The mbo operon maintains synteny and is inserted in the same genomic location, with high sequence conservation around the insertion point, for all the strains in groups I and II. These data support the idea that the mbo operon was acquired horizontally and only once by the ancestor of groups I and II from genomospecies 1 within the P. syringae complex.en
dc.description.sponsorshipThis work was supported by grants from the regional government of Andalucía (Spain), grants from CICE-Junta de Andalucía, Ayudas Grupo PAIDI AGR-169, and Proyecto de Excelencia (P07-AGR-02471) and Plan Nacional IDI grant AGL2008-55311-CO2-01 (Ministerio de Ciencia e Innovación), cofinanced by FEDER (European Union). Plan Propio of the University of Málaga funded a stay by V.J.C. at the Universidad Pública de Navarra, Spain.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiology 2013, 79 (3)en
dc.rights© 2013 American Society for Microbiology. All Rights Reserveden
dc.subjectMangotoxinen
dc.subjectPhylogenyen
dc.subjectDiagnostic PCRen
dc.subjectAntimetabolite toxinsen
dc.subjectVirulenceen
dc.subjectMango apical necrosisen
dc.subjectLateral gene transferen
dc.titleThe mangotoxin biosynthetic operon (mbo) is specifically distributed within Pseudomonas syringae genomospecies 1 and was acquired only once during evolutionen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1128/AEM.03007-12
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/AGL2008-55311
dc.relation.publisherversionhttps://dx.doi.org/10.1128/AEM.03007-12
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoa


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record