Telomere organization in the ligninolytic basidiomycete Pleurotus ostreatus
Fecha
2008Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1128/aem.01889-08
Resumen
Telomeres are structural and functional chromosome regions that are essential for the cell cycle to proceed
normally. They are, however, difficult to map genetically and to identify in genome-wide sequence programs
because of their structure and repetitive nature. We studied the telomeric and subtelomeric organization in the
basidiomycete Pleurotus ostreatus using a combination of molecular and b ...
[++]
Telomeres are structural and functional chromosome regions that are essential for the cell cycle to proceed
normally. They are, however, difficult to map genetically and to identify in genome-wide sequence programs
because of their structure and repetitive nature. We studied the telomeric and subtelomeric organization in the
basidiomycete Pleurotus ostreatus using a combination of molecular and bioinformatics tools that permitted us
to determine 19 out of the 22 telomeres expected in this fungus. The telomeric repeating unit in P. ostreatus is
TTAGGG, and the numbers of repetitions of this unit range between 25 and 150. The mapping of the telomere
restriction fragments to linkage groups 6 and 7 revealed polymorphisms compatible with those observed by
pulsed field gel electrophoresis separation of the corresponding chromosomes. The subtelomeric regions in
Pleurotus contain genes similar to those described in other eukaryotic systems. The presence of a cluster of
laccase genes in chromosome 6 and a bipartite structure containing a Het-related protein and an alcohol
dehydrogenase are especially relevant; this bipartite structure is characteristic of the Pezizomycotina fungi
Neurospora crassa and Aspergillus terreus. As far as we know, this is the first report describing the presence of
such structures in basidiomycetes and the location of a laccase gene cluster in the subtelomeric region, where,
among others, species-specific genes allowing the organism to adapt rapidly to the environment usually map. [--]
Materias
Pleurotus ostreatus,
Telomere organization
Editor
American Society for Microbiology
Publicado en
Applied and Environmental Microbiology, Mar. 2009, Vol. 75, No. 5, p. 1427–1436
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
Versión del editor
Entidades Financiadoras
This work has been supported by funds from the AGL2005-08005-
C02-01 and GEN2006-27843-E grants and by additional institutional
support from the Public University of Navarre.