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Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures
dc.creator | Castanera Andrés, Raúl | es_ES |
dc.creator | Pérez Garrido, María Gumersinda | es_ES |
dc.creator | Omarini, Alejandra | es_ES |
dc.creator | Alfaro Sánchez, Manuel | es_ES |
dc.creator | Pisabarro de Lucas, Gerardo | es_ES |
dc.creator | Faraco, Vicenza | es_ES |
dc.creator | Amore, Antonella | es_ES |
dc.creator | Ramírez Nasto, Lucía | es_ES |
dc.date.accessioned | 2019-01-10T08:27:58Z | |
dc.date.available | 2019-01-10T08:27:58Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0099-2240 (Print) | |
dc.identifier.issn | 1098-5336 (Electronic) | |
dc.identifier.uri | https://hdl.handle.net/2454/31946 | |
dc.description.abstract | The genome of the white rot basidiomycete Pleurotus ostreatus includes 12 phenol oxidase (laccase) genes. In this study, we examined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription- quantitative PCR (RT-qPCR)-based approach and focused on determining the reaction parameters (in particular, the reference gene set for the normalization and reaction efficiency determinations) used to achieve an accurate estimation of the relative gene expression values. The results suggested that (i) laccase gene transcription is upregulated in the induced submerged fermentation (iSmF) cultures but downregulated in the solid fermentation (SSF) cultures, (ii) the Lacc2 and Lacc10 genes are the main sources of laccase activity in the iSmF cultures upon induction with water-soluble wheat straw extracts, and (iii) an additional, as-yet-uncharacterized activity (Unk1) is specifically induced in SSF cultures that complements the activity of Lacc2 and Lacc10. Moreover, both the enzymatic laccase activities and the Lacc gene family transcription profiles greatly differ between closely related strains. These differences can be targeted for biotechnological breeding programs for enzyme production in submerged fermentation reactors. | en |
dc.description.sponsorship | This work was supported by funds from project AGL2008-05608-C02-01 of the Spanish National Research Plan, from Bioethanol-Euroinnova, and from the Autonomous Government of Navarre and by additional institutional support from the Public University of Navarre. | en |
dc.format.extent | 9 p. | |
dc.format.mimetype | application/pdf | en |
dc.format.mimetype | application/zip | en |
dc.language.iso | eng | en |
dc.publisher | American Society for Microbiology | en |
dc.relation.ispartof | Applied and Environmental Microbiology, June 2012 Volume 78 Number 11, p. 4037–4045 | en |
dc.rights | © 2012, American Society for Microbiology. All Rights Reserved. | en |
dc.subject | Pleurotus ostreatus | en |
dc.subject | Submerged fermentation | en |
dc.subject | Solid fermentation | en |
dc.subject | Laccase gene transcription | en |
dc.title | Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures | en |
dc.type | info:eu-repo/semantics/article | en |
dc.type | Artículo / Artikulua | es |
dc.contributor.department | Producción Agraria | es_ES |
dc.contributor.department | Nekazaritza Ekoizpena | eu |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
dc.identifier.doi | 10.1128/aem.07880-11 | |
dc.relation.publisherversion | https://doi.org/10.1128/aem.07880-11 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.type.version | Versión publicada / Argitaratu den bertsioa | es |
dc.contributor.funder | Gobierno de Navarra / Nafarroako Gobernua | es |
dc.contributor.funder | Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa | es |