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dc.creatorCantón, Pablo Emilianoes_ES
dc.creatorZanicthe Reyes, Esmeraldaes_ES
dc.creatorRuiz de Escudero Fuentemilla, Íñigoes_ES
dc.creatorBravo, Alejandraes_ES
dc.creatorSoberón, Marioes_ES
dc.identifier.issn0196-9781 (Print)
dc.identifier.issn1873-5169 (Electronic)
dc.description.abstractBacillus thuringiensis subsp. israelensis (Bti) produces at least four different crystal proteins that are specifically toxic to different mosquito species and that belong to two non-related family of toxins, Cry and Cyt named Cry4Aa, Cry4Ba, Cry11Aa and Cyt1Aa. Cyt1Aa enhances the activity of Cry4Aa, Cry4Ba or Cry11Aa and overcomes resistance of Culex quinquefasciatus populations resistant to Cry11Aa, Cry4Aa or Cry4Ba. Cyt1Aa synergized Cry11Aa by their specific interaction since single point mutants on both Cyt1Aa and Cry11Aa that affected their binding interaction affected their synergistic insecticidal activity. In this work we show that Cyt1Aa loop 6–E K198A, E204A and 7 K225A mutants affected binding and synergism with Cry4Ba. In addition, site directed mutagenesis showed that Cry4Ba domain II loop -8 is involved in binding and in synergism with Cyt1Aa since Cry4Ba SI303-304AA double mutant showed decreased binding and synergism with Cyt1Aa. These data suggest that similarly to the synergism between Cry11Aa and Cyt1Aa toxins, the Cyt1Aa also functions as a receptor for Cry4Ba explaining the mechanism of synergism between these two Bti toxins.en
dc.description.sponsorshipResearch was funded in part through grants from the National Institutes of Health, 1R01 AI066014, DGAPA/UNAM IN218608 and IN210208-N, CONACyT U48631-Q. IRdE received a José Castillejo postdoctoral grant (Spanish Ministry of Education and Science), and a grant for mobility for Teaching and Research Staff of Public University of Navarre, Spain (UPNA).en
dc.format.extent14 p.
dc.relation.ispartofPeptides 32 (2011) 595–600en
dc.rights© 2010 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0.en
dc.subjectBacillus thuringiensisen
dc.subjectCry toxinsen
dc.subjectCyt toxinsen
dc.titleBinding of Bacillus thuringiensis subsp. israelensis Cry4Ba to Cyt1Aa has an important role in synergismen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentIdAB – Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.type.versionVersión aceptada / Onetsi den bertsioaes
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes

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© 2010 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0.
Except where otherwise noted, this item's license is described as © 2010 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0.