Extraction of phenolic compounds from populus salicaceae bark
Fecha
2022Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Identificador del proyecto
Impacto
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10.3390/biom12040539
Resumen
Lignocellulosic residues have the potential for obtaining high value-added products that could be better valorized if biorefinery strategies are adopted. The debarking of short-rotation crops yields important amounts of residues that are currently underexploited as low-grade fuel
and could be a renewable source of phenolic compounds and other important phytochemicals.
The isolation of these com ...
[++]
Lignocellulosic residues have the potential for obtaining high value-added products that could be better valorized if biorefinery strategies are adopted. The debarking of short-rotation crops yields important amounts of residues that are currently underexploited as low-grade fuel
and could be a renewable source of phenolic compounds and other important phytochemicals.
The isolation of these compounds can be carried out by different methods, but for attaining an
integral valorization of barks, a preliminary extraction step for phytochemicals should be included. Using optimized extraction methods based on Soxhlet extraction can be effective for the isolation of phenolic compounds with antioxidant properties. In this study, poplar bark (Populus Salicaceae)
was used to obtain a series of extracts using five different solvents in a sequential extraction of 24 h
each in a Soxhlet extractor. Selected solvents were put in contact with the bark sample raffinate
following an increasing order of polarity: n-hexane, dichloromethane, ethyl acetate, methanol, and
water. The oily residues of the extracts obtained after each extraction were further subjected to flash
chromatography, and the fractions obtained were characterized by gas chromatography coupled
with mass spectrometry (GC–MS). The total phenolic content (TPC) was determined using the
Folin–Ciocalteu method, and the antioxidant activity (AOA) of the samples was evaluated in their
reaction with the free radical 2,2-Diphenyl-picrylhydrazyl (DPPH method). Polar solvents allowed for higher individual extraction yields, with overall extraction yields at around 23% (dry, ash-free
basis). Different compounds were identified, including hydrolyzable tannins, phenolic monomers
such as catechol and vanillin, pentoses and hexoses, and other organic compounds such as long-chain alkanes, alcohols, and carboxylic acids, among others. An excellent correlation was found between TPC and antioxidant activity for the samples analyzed. The fractions obtained using methanol showed the highest phenolic content (608 g of gallic acid equivalent (GAE)/mg) and the greatest antioxidant activity. [--]
Materias
Antioxidants,
Tannins,
Phenolics,
Soxhlet,
Folin-Ciocalteu,
2,2-Diphenyl-picrylhydrazyl,
DPPH
Editor
MDPI
Publicado en
Biomolecules, 2022, 12 (4), p. 539
Departamento
Universidad Pública de Navarra. Departamento de Ciencias /
Nafarroako Unibertsitate Publikoa. Zientziak Saila /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute for Advanced Materials and Mathematics - INAMAT2
Versión del editor
Entidades Financiadoras
This research was funded by the Gobierno de Navarra, grant number PC177-178 Reducenano 2.0 and Spanish Ministry of Science (AbFine, PID2020-114936RB-I00). The APC was funded by the Universidad Pública de Navarra (UPNA).