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dc.creatorFarrán Blanch, Inmaculadaes_ES
dc.creatorFernández San Millán, Aliciaes_ES
dc.creatorAncín Rípodas, Maríaes_ES
dc.creatorLarraya Reta, Luis Maríaes_ES
dc.creatorVeramendi Charola, Jones_ES
dc.date.accessioned2018-04-10T12:46:02Z
dc.date.available2018-04-10T12:46:02Z
dc.date.issued2014
dc.identifier.issn1380-3743 (Print)
dc.identifier.issn1572-9788 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/28228
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in Molecular Breeding. The final authenticated version is available online at: http://dx.doi.org/10.1007/s11032-014-0047-x.en
dc.description.abstractBioethanol is mainly produced from food crops such as sugar cane and maize while it has been held partly responsible for the rise of food commodity prices. Tobacco, integrated in biorefinery facilities for the extraction of different compounds, could turn into an alternative feedstock for biofuel production. When grown for energy production, using high plant densities and several mowings during the growing season, tobacco can produce large amounts of inexpensive green biomass. We have bred two commercial tobacco cultivars (Virginia Gold and Havana 503B) to increment the carbohydrate content by the overexpression of thioredoxin f in the chloroplast. Marker-free transplastomic plants were rescued and their agronomic performance under field conditions was evaluated. These plants were phenotypically equivalent to their wild types yet showed increased starch (up to 280%) and soluble sugar (up to 74%) contents in leaves relative to their control plants. Fermentable sugars released from the stalk were also higher (up to 24%) for transplastomic plants. After a heat pretreatment, enzymatic hydrolysis and yeast fermentation of leaf and stalk hydrolysates, an average of 20-40% more ethanol was obtained from transplastomic plants in relation to their control wild types. We propose an integral exploitation of the entire tobacco plant managed as a forage crop (harvesting sugar and starch-rich leaves and lignocellulosic stalks) that could considerably cheapen the entire production process.en
dc.description.sponsorshipThis work was supported by Grant AGL2010-15107 from the Ministerio de Ciencia e Innovación (Spain).en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherSpringeren
dc.relation.ispartofMolecular Breeding, 2014, Vol. 34, Issue 2, pp 457–469en
dc.rights© Springer Science+Business Media Dordrecht 2014en
dc.subjectTobaccoen
dc.subjectChloroplast transformationen
dc.subjectThioredoxinen
dc.subjectBioethanolen
dc.subjectField trialen
dc.subjectCarbohydrateen
dc.titleIncreased bioethanol production from commercial tobacco cultivars overexpressing thioredoxin f grown under field conditionsen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.departmentIdAB - Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1007/s11032-014-0047-x
dc.relation.publisherversionhttps://doi.org/10.1007/s11032-014-0047-x
dc.type.versionVersión aceptada / Onetsi den bertsioaes
dc.type.versioninfo:eu-repo/semantics/acceptedVersionen


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